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Immunohistochemistry Protocols

Staining Protocols

  • GFAP, Iba1 with Hoeschst Counterstain
  • Ki-67: Marker for enodgenous cell proliferation
    • See: Gil-Mohapel, J., et al. 2013. Hippocampal neurogenesis levels predict WATERMAZE search strategies in the aging brain.
  • NeuroD: Marker for neuronal differentiation 
    • See: Simpson, JM., et al. 2011. Altered adult hippocampal neurogenesis in the YAC128 transgenic mouse model of Huntington disease.
  • Doublecortin: Marker for differentiated and migrating neuroblasts
    • See: Simpson, JM., et al. 2011. Altered adult hippocampal neurogenesis in the YAC128 transgenic mouse model of Huntington disease.
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