Tool list
- Fumehoods
- Bench space for experimental set up
- Scales and balances (micro and gross)
- Sonicating water bath
- Probe tip sonicator
- Micropipets, serological pipets, and multi channel pipets (both standard and electronic)
- Microfuges
- Sonicators:
- Lyophilizer
- pH meter(s)
- Microwave
- Hybridization oven
- Heat/Stir plates
- All standard laboratory glassware
- Flammables storage
- Distilled water
- Dedicated Biosafety Level 2 (BSL-2) and Human Pathogens & Toxins Act (HPTA) certified space
- Class IIA Biological Safety Cabinet
- CytoFLEX Flow Cytometer (Beckman Coulter)
- 3 lasers, 6 colours, 8 parameters, 13 dye band-pass filters
- Violet 405nm , Blue 488nm, Red 638nm with FSC and SSC.
- 96 well plate loader module with mode control switch
- Bright field and Fluorescence Imager (Cytation 5, Biotek)
- Live cell imaging with CO2 and incubator functions
- 25x apo, 4x, 20x, 60x objectives
- Adaptors that can image T-25 plates, Petrie dishes, 96,48, 24, and 12 well plates, and slides
- DAPI, GFP, Texas Red
- 3D Tissue Printer (Celllink)
- CO2 Incubators
- Pipette controllers for serological, plastic, single-use, pipets
- Dedicated micropipets
- Water bath
- Frosty (for preparing cryo storage of cell lines)
- Automatic cell counter (Countess, Invitrogen)
- Inverted Tissue Culture Microscope (10x – 40x)
- Water bath
- Cold and Cryogenic Storage
- Fridge, -20°C, -80 °C, LN2
- Dedicated Biosafety Level 2 (BSL-2) and Human Pathogens & Toxins Act (HPTA) certified space
- Class IIA Biological Safety Cabinet
- Multiple temperature controlled Incubators
- Shaking, non-shaking, variable temperatures, up to 20 L at a time
- Optical Density Meter (OD 600 for Bacterial culture)
- Bacterial culture glassware
- Culture tubes, range of baffled flasks from 125 ml to 2000 ml
- Cold and Cryogenic Storage
- Fridge, -20°C, -80 °C, LN2
- Bench top Autoclave
- Polymerase Chain Reaction Thermocyclers
- Standard thermocycler
- Quantitative (qPCR) QIAquant 5 plex (Qiagen)
- Digital (dPCR) QUIAcuity One 5 plex (Qiagen)
- PowerLyzer Tissue disruptor/bead mill (24 sample)
- RNA-Only Room
- Separate, authorized-use only access
- Dedicated RNA-Only use equipment with gel running and extraction equipment
- Magnet stands for bead pull downs
- Vortex Mixers
- Standard multi-speed (tubes, plates, and flat top heads)
- Mini (Eppendorf tubes)
- Centrifuges
- Room temp and refrigerated
- Plates
- Eppendorf tubes, Falcon tubes, 50 ml, 500 ml, 1000 ml
- Low speed and high speed
- Sorvall Lynx (68,905 x g and up to 4L capacity, with refrigeration)
- Agarose Gel casting trays and running apparatus
- Agarose Gel imager
- Stock solutions for Gels
- 2ul Nucleic acid quantification spectrophotometer (8 samples +2 blanks at a time)
- Micropipettes – Manual, Single Channel and Multi-channel
- P1, 2, 10, 20, 200, and 1000
- Electronic Micropipettes – Programmable Single Channel and Multi-channel
- Micropipette Tips (Sterile & Non-Sterile)
- Eppendorf tubes (Sterile & Non-Sterile)
- Pipettes – sterile
- Peptide synthesizer (MultiPep-1, CEM)
- 8-column, 96-well, and SPOT array modules
- Cloning infrastructure
- Bacterial Expression infrastructure
- Probe tip sonicator for cell lysis
- Large scale refrigerated centrifuge (4x 1L and 8x 50 ml up to 68,905 x g)
- Large scale shaking-heating-cooling incubators with glassware
- Polyacrylamide gel casting and running apparatus (up to 4 gels at a time)
- Gel Staining trays and drying frames
- Western Blot apparatus
- Western Blot stock buffer solutions
- Dialysis equipment
- 2ul protein quantification spectrophotometer (8 samples +2 blanks at a time)
- Fluorescence Imaging Plate Reader that can quantify fluorescent and absorbance output on imaging samples (Cytation 5, Biotek)
- 2 Multimode Plate Readers (Cytation 5, Biotek and SpectraMax M5, Molecular Devices)
- 96 well plates, 384 well plates , cuvettes, and 2 ul samples
- Full spectral scan
- UV-Vis absorbance
- Fluorescence Polarization
- FRET & TR-FRET
- FLINT
- Kinetic, endpoint, scanning
- including excitation scans with fixed emission and fixed emission with excitation scan
- Robotics liquid handler for in-instrument dispensing into plates (for setting up calibration curves or checkerboards and for reaction monitoring)
- Fluorescence Imaging Plate Reader that can quantify fluorescent and absorbance output on imaging samples
- Surface Plasmon Resonance Biacore X100 (GE)
- Biolayer Interferometry (Blitz, Sartorius) for measuring binding kinetics